a 10042  (Thermo Fisher)

Journal: Clinical Oral Investigations

Article Title: Cytokine expression of soft tissue cells cultured with titanium discs and their respective supernatants in vitro

doi: 10.1007/s00784-024-06123-1

Figure Lengend Snippet: Workflow represents the sample distribution of 216 titanium discs in three groups of surface treatment: SLA, SBF and SBF + ODN. The SLA was considered as a control group. The diagram describes the analyses performed and the respective exposure times for each experiment

Article Snippet: The discs were immersed and incubated in SBF containing either no or 260 µg/mL of odanacatib (ODN, MedChemExpress LLC, Monmouth Junction, NJ) at pH 7.25 and 37 °C for 4 days.

Techniques: Control

Journal: Clinical Oral Investigations

Article Title: Cytokine expression of soft tissue cells cultured with titanium discs and their respective supernatants in vitro

doi: 10.1007/s00784-024-06123-1

Figure Lengend Snippet: Scanning electron microscopy (SEM) images of the surfaces of SLA, SBF, and SBF + ODN discs at 10,000 × magnification. A - D SLA shows uniform microroughness with varying depths and sizes. E – H SBF exhibits the expected spider web-like pores of the hydroxyapatite coating. I - L SBF + ODN displays a rough texture with globular microcavities, interlocking pores, and nanometric particles. Obviously, the presence of ODN affects the morphology of the hydroxyapatite coating compared to the SBF alone discs – and its wettability

Article Snippet: The discs were immersed and incubated in SBF containing either no or 260 µg/mL of odanacatib (ODN, MedChemExpress LLC, Monmouth Junction, NJ) at pH 7.25 and 37 °C for 4 days.

Techniques: Electron Microscopy

Journal: Clinical Oral Investigations

Article Title: Cytokine expression of soft tissue cells cultured with titanium discs and their respective supernatants in vitro

doi: 10.1007/s00784-024-06123-1

Figure Lengend Snippet: Energy-dispersive X-ray (EDX) spectroscopy of the surfaces of SLA, SBF, and SBF + ODN discs, showing the apparent concentration of chemical elements present on the surface

Article Snippet: The discs were immersed and incubated in SBF containing either no or 260 µg/mL of odanacatib (ODN, MedChemExpress LLC, Monmouth Junction, NJ) at pH 7.25 and 37 °C for 4 days.

Techniques: Spectroscopy, Concentration Assay

Journal: Clinical Oral Investigations

Article Title: Cytokine expression of soft tissue cells cultured with titanium discs and their respective supernatants in vitro

doi: 10.1007/s00784-024-06123-1

Figure Lengend Snippet: Variation in contact angle values (θ) in different surface modifications

Article Snippet: The discs were immersed and incubated in SBF containing either no or 260 µg/mL of odanacatib (ODN, MedChemExpress LLC, Monmouth Junction, NJ) at pH 7.25 and 37 °C for 4 days.

Techniques:

Journal: Clinical Oral Investigations

Article Title: Cytokine expression of soft tissue cells cultured with titanium discs and their respective supernatants in vitro

doi: 10.1007/s00784-024-06123-1

Figure Lengend Snippet: Surface roughness was evaluated by measuring Ra ( A ) and Rz ( B ) values for each group SLA, SBF and SBF + ODN. The results showed that the ODN coating significantly increased surface roughness compared to SBF alone. Different symbol shapes represent mean values of independent readings. Statistical analysis was performed using ratio-paired t-tests compared to untreated controls, and p-values are shown

Article Snippet: The discs were immersed and incubated in SBF containing either no or 260 µg/mL of odanacatib (ODN, MedChemExpress LLC, Monmouth Junction, NJ) at pH 7.25 and 37 °C for 4 days.

Techniques:

Journal: Clinical Oral Investigations

Article Title: Cytokine expression of soft tissue cells cultured with titanium discs and their respective supernatants in vitro

doi: 10.1007/s00784-024-06123-1

Figure Lengend Snippet: Cell viability assays were performed on human gingival fibroblasts and HSC2 cells stimulated with the supernatants and discs from each group—SLA, SBF, and SBF + ODN overnight

Article Snippet: The discs were immersed and incubated in SBF containing either no or 260 µg/mL of odanacatib (ODN, MedChemExpress LLC, Monmouth Junction, NJ) at pH 7.25 and 37 °C for 4 days.

Techniques:

Journal: Clinical Oral Investigations

Article Title: Cytokine expression of soft tissue cells cultured with titanium discs and their respective supernatants in vitro

doi: 10.1007/s00784-024-06123-1

Figure Lengend Snippet: Cathepsin K activity was conducted with titanium discs coated with SBF and SBF + ODN, in the presence of osteoclast lysates. Fluorescence intensity indicating CATK activity was determined. Statistical analysis was performed using ratio-paired t-tests. Notably, the presence of ODN significantly lowers the CATK activity intrinsic to the osteoclast lysate

Article Snippet: The discs were immersed and incubated in SBF containing either no or 260 µg/mL of odanacatib (ODN, MedChemExpress LLC, Monmouth Junction, NJ) at pH 7.25 and 37 °C for 4 days.

Techniques: Activity Assay, Fluorescence

Journal: Clinical Oral Investigations

Article Title: Cytokine expression of soft tissue cells cultured with titanium discs and their respective supernatants in vitro

doi: 10.1007/s00784-024-06123-1

Figure Lengend Snippet: Bone marrow-derived cells were exposed to supernatants ( A , C ) and titanium discs ( B , C ) from each group in the presence of M-CSF, RANKL, and TGF-β (MRT). A , B Gene expression levels of TRAP and CTSK were normalized to untreated cells. Different symbol shapes in the graphs represents independent experiments. The SBF coating resulted in decreased TRAP and CTSK expression, but this was not reflected in TRAP staining. Statistical analysis was performed using ratio-paired t-tests, with p-values indicated. C Representative TRAP-stained images show that osteoclast formation was not significantly suppressed by SLA, SBF, or SBF + ODN disc and respective supernatants

Article Snippet: The discs were immersed and incubated in SBF containing either no or 260 µg/mL of odanacatib (ODN, MedChemExpress LLC, Monmouth Junction, NJ) at pH 7.25 and 37 °C for 4 days.

Techniques: Derivative Assay, Expressing, Staining